RT info:eu-repo/semantics/article
T1 Molecular identification of Helicoverpa armigera (Noctuidae: Heliothinae) in Amambay Department, Paraguay
T2 Identificación molecular de Helicoverpa armígera (Noctuidae: Heliothinae) en el departamento de Amambay, Paraguay
A1 Ferreira Aguero, Marcos Arturo
A1 Gusman Sosa, Enson
A1 Arias Ruiz Díaz, Osmar René
AB The Helicoverpa armigera (Noctuidae: Heliothinae) is a polyphagous and cosmopolitan pest distributed throughout South America. The objective of this research was to identify by PCR-RFLP molecular analysis, the presence of H. armigera in the Amambay region in Paraguay. Delta traps with sexual pheromone were deployed in the 2016/2017 season soybean crop distributed at the following locations: Pedro Juan Caballero, Zanja Pytã, Capitán Bado, Karapã’ĩ and Bella Vista Norte. Sampling cloth was used for collecting caterpillars. The collected specimens were sorted by external morphological characters, individualized in labeled jars containing pure alcohol. 24 samples were sent to the Arthropods Molecular Ecology Laboratory ESALQ/USP for identification by PCR-RFLP molecular analysis. Ten specimens of H. armigera belonging to Fortuna and Zanja Pytã were identified. Ten other specimens were identified as H. zea belonging to Cerro Cora'i, Captain Bado, Cerro Kuatiá and Karapã’ĩ. The remaining four samples did not amplify by PCR-RFLP. The analysis revealed the occurrence of H. armigera in soybean and maize crops in the northern region of Paraguay.
PB Universidad Nacional de Asunción. Facultad de Ciencias Agrarias
SN 1684-9086
YR 2018
FD 2018-07-31
LK http://hdl.handle.net/20.500.14066/2803
UL http://hdl.handle.net/20.500.14066/2803
LA eng
NO Ferreira Aguero, M. A., Gusman Sosa, E., & Arias, O. R. (2018). Molecular identification of Helicoverpa armigera (Noctuidae: Heliothinae) in Amambay Department, Paraguay [Identificación molecular de Helicoverpa armígera (Noctuidae: Heliothinae) en el departamento de Amambay, Paraguay]. Investigación Agraria, 20(1), 84-90. https://doi.org/10.18004/investig.agrar.2018.junio.84-90
NO Correspondence author: ingeniero.ferreira1@gmail.com
NO Sección: Notas de Investigación
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 21-may-2026