RT Journal Article
T1 Evaluation of p53 Immunohistochemical Expression Using Open-Source Software for Digital Image Analysis. A Tissue Microarray Study of Penile Squamous Cell Carcinomas.
A1 Chaux Salinas, Alcides
A1 Burnett, Authur
A1 Netto, George
A2 Centro para el Desarrollo de la Investigación Científica - CEDIC (PY)
AB The addition of molecular biomarkers is needed to increase the accuracy of pathologic factors as prognosticators of outcome in penile squamous cell carcinomas (SCC). Evaluation of these biomarkers is usually carried out by immunohistochemistry. Herein we assess p53 immunohistochemical expression on tissue samples of penile SCC using freely-available, open-source software packages for digital image analysis. We also compared the results of digital analysis with standard visual estimation. Percentages of p53 positive cells were higher by visual estimation than by digital analysis. However, correlation was high between both methods. Our study shows that evaluation of p53 immunohistochemical expression is feasible using open-source software packages for digital image analysis. Although our analysis was limited to penile SCC, the rationale should also hold for other tumor types in which evaluation of p53 immunohistochemical expression is required. This approach would reduce interobserver variability, and would provide a standardized method for reporting the results of immunohistochemical stains. As these diagnostic tools are freely-available online, researchers and practicing pathologists could incorporate them in their daily practice without increasing diagnostic costs.
YR 2015
FD 2015
LK http://hdl.handle.net/20.500.14066/3579
UL http://hdl.handle.net/20.500.14066/3579
LA eng
NO CONACYT - Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 03-nov-2024