RT Journal Article
T1 Serum biomarkers and anti-flavivirus antibodies at presentation as indicators of severe dengue
A1 Bernal, Cynthia
A1 Ping, Sara
A1 Rojas Segovia, Alejandra María
A1 Caballero, Oliver
A1 Stittleburg, Victoria
A1 Arévalo de Guillén, Yvalena
A1 Langjahr Penayo, Patricia Elena
A1 Pinsky, Benjamin A.
A1 Von-Horoch, Marta
A1 Luraschi, Patricia
A1 Cabral, Sandra
A1 Sánchez, María Cecilia
A1 Torres, Aurelia
A1 Cardozo, Fátima
A1 Waggoner, Jesse J.
A2 Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud
AB Background. Dengue is the most common vector-borne viral disease worldwide. Most cases are mild, but some evolve into severe dengue (SD), with high lethality. Therefore, it is important to identify biomarkers of severe disease to improve outcomes and judiciously utilize resources.Methods/Principal findings. One hundred forty-five confirmed dengue cases (median age, 42; range <1–91 years), enrolled from February 2018 to March 2020, were selected from an ongoing study of suspected arboviral infections in metropolitan Asunción, Paraguay. Cases included dengue virus types 1, 2, and 4, and severity was categorized according to the 2009 World Health Organization guidelines. Testing for anti-dengue virus IgM and IgG and serum biomarkers (lipopolysaccharide binding protein and chymase) was performed on acute-phase sera in plate-based ELISAs; in addition, a multiplex ELISA platform was used to measure anti-dengue virus and anti-Zika virus IgM and IgG. Complete blood counts and chemistries were performed at the discretion of the care team. Age, gender, and pre-existing comorbidities were associated with SD vs. dengue with/without warning signs in logistic regression with odds ratios (ORs) of 1.07 (per year; 95% confidence interval, 1.03, 1.11), 0.20 (female; 0.05,0.77), and 2.09 (presence; 1.26, 3.48) respectively. In binary logistic regression, for every unit increase in anti-DENV IgG in the multiplex platform, odds of SD increased by 2.54 (1.19–5.42). Platelet count, lymphocyte percent, and elevated chymase were associated with SD in a combined logistic regression model with ORs of 0.99 (1,000/μL; 0.98,0.999), 0.92 (%; 0.86,0.98), and 1.17 (mg/mL; 1.03,1.33) respectively.Conclusions. Multiple, readily available factors were associated with SD in this population. These findings will aid in the early detection of potentially severe dengue cases and inform the development of new prognostics for use in acute-phase and serial samples from dengue cases.
PB Public Library of Science
SN 1935-2727
YR 2023
FD 2023-02-27
LK http://hdl.handle.net/20.500.14066/4318
UL http://hdl.handle.net/20.500.14066/4318
LA eng
NO Bernal C, Ping S, Rojas A, Caballero O, Stittleburg V, de Guillén Y, et al. (2023) Serum biomarkers and anti-flavivirus antibodies at presentation as indicators of severe dengue. PLoS Negl Trop Dis 17(2): e0010750. https://doi.org/10.1371/journal.pntd.0010750
NO Corresponding author: fati.cardozo@hotmail.com (FC); jjwaggo@emory.edu (JJW)
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 09-nov-2024