RT info:eu-repo/semantics/article
T1 Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay
A1 Brítez López, Sady Camila
A1 Herrera Cabrera, Leidi Maira
A1 Ferreira, María C.
A1 Rolón, Leticia M.
A1 Ruíz, Vanessa
A1 González Brítez, Nilsa Elizabeth
A2 Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud
A2 Asociación de FuncionariosDocentes e Investigadores de la Universidad Nacional de Caaguazú
AB Objective: To determine the genetic diversity of Aedes aegypti in the Central-Alto Paraná cross-border road corridor of Paraguay, an area that has reports of dengue cases. Materials and methods: Twenty adult females were selected from hatching Ae. aegypti eggs from households geolocated in the departments of Alto Paraná, Caaguazú, Cordillera and Central, between 2018 and 2019. DNA was extracted from the tissue of females for amplifying their polymorphic patterns by random amplification of polymorphic DNA by PCR (RAPD-PCR), using primers H3 and B03 in order to identify genetic parameters of population diversity. The relationships between mosquito populations according to locality were observed by unpaired arithmetic mean analysis. We used DIVA-GIS 7.3.0 and MAXENT to analyze the suitable areas of actual and potential geographic distribution of these Ae. aegypti populations. Results: Forty loci were identified by RAPD-PCR profiling, with moderate gene differentiation (Gst = 0.12). The cross-border corridor presented bioclimatic conditions for the presence of variant populations of Ae. aegypti, with precipitation in the warmest quarter and mean temperature in the driest quarter being determinant in the distribution. Conclusions: There is evidence of moderate genetic diversity in Ae. aegypti populations from areas that have reported dengue cases in the cross-border road corridor linking the Central and Alto Paraná departments of Paraguay. The study of genetic variability of Ae. aegypti is very useful for entomo-epidemiological surveillance and evaluation of possible resistance to chemical control.
PB Instituto Nacional de Salud
SN 1726-4634
YR 2022
FD 2022-06-30
LK http://hdl.handle.net/20.500.14066/4374
UL http://hdl.handle.net/20.500.14066/4374
LA eng
NO Brítez, S. C., Herrera, L., Ferreira, M. C., Rolón, L. M., Ruiz, V., & González Brítez, N. (2022). Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay. Revista Peruana de Medicina Experimental y Salud Pública, 39(2), 170-7. https://doi.org/10.17843/rpmesp.2022.392.10709
NO Correspondence: Nilsa González Brítez; gbritez.nilsa@gmail.com
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 03-nov-2024