RT info:eu-repo/semantics/article
T1 Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children
A1 Guillén Fretes, Rosa María
A1 Salinas, Claudia
A1 Mendoza Álvarez, Alejandro
A1 Rubio Rodríguez, Luis A.
A1 Díaz de Usera, Ana
A1 Lorenzo Salazar, José M.
A1 González Montelongo, Rafaela
A1 Flores, Carlos
A1 Rodríguez Acosta, Fátima
A2 VignoliRafael
AB Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major human pathogens. It could carry numerous resistance genes and virulence factors in its genome, some of which are related to the severity of the infection. An observational, descriptive, cross-sectional study was designed to molecularly analyze MRSA isolates that cause invasive infections in Paraguayan children from 2009 to 2013. Ten representative MRSA isolates of the main clonal complex identified were analyzed with short-read paired-end sequencing and assessed for the virulome, resistome, and phylogenetic relationships. All the genetically linked MRSA isolates were recovered from diverse clinical sources, patients, and hospitals at broad gap periods. The pan-genomic analysis of these clones revealed three major and different clonal complexes (CC30, CC5, and CC8), each composed of clones closely related to each other. The CC30 genomes prove to be a successful clone, strongly installed and disseminated throughout our country, and closely related to other CC30 public genomes from the region and the world. The CC5 shows the highest genetic variability, and the CC8 carried the complete arginine catabolic mobile element (ACME), closely related to the USA300-NAE-ACME+, identified as the major cause of CA-MRSA infections in North America. Multiple virulence and resistance genes were identified for the first time in this study, highlighting the complex virulence profiles of MRSA circulating in the country. This study opens a wide range of new possibilities for future projects and trials to improve the existing knowledge on the epidemiology of MRSA circulating in Paraguay.
PB American Society for Microbiology
YR 2024
FD 2024-02-28
LK http://hdl.handle.net/20.500.14066/4390
UL http://hdl.handle.net/20.500.14066/4390
LA eng
NO Guillén, R., Salinas, C., Mendoza Álvarez, A., Rubio Rodríguez, L. A., Díaz de Usera, A., Lorenzo Salazar, J. M., González Montelongo, R., Flores, C., & Rodríguez, F. (2024). Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children. Microbiology Spectrum, e03012-23. https://doi.org/10.1128/spectrum.03012-23
NO Address correspondence to Fátima Rodríguez,frodriguez@iics.una.py
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 14-may-2024