RT info:eu-repo/semantics/article
T1 Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
A1 Cebrián, Jorge
A1 Martínez, Víctor
A1 Hernández, Pablo
A1 Krimer, Dora B.
A1 Martínez Robles, María Luisa
A1 Schvartzman, Jorge B.
A1 Fernández de Nestosa, María José
AB Two-dimensional (2D) agarose gel electrophoresis is the method of choice to analyze DNA topology. The possibility to use E. coli strains with different genetic backgrounds in combination with nicking enzymes and different concentrations of norfloxacin improves the resolution of 2D gels to study the electrophoretic behavior of three different families of DNA topoisomers: supercoiled DNA molecules, post-replicative catenanes, and knotted DNA molecules. Here, we describe the materials and procedures required to optimize their separation by 2D gels. Understanding the differences in their electrophoretic behavior can help explain some important physical characteristics of these different types of DNA topoisomers.
PB Bio-protocol LLC
SN 2331-8325
YR 2024
FD 2024-05-05
LK http://hdl.handle.net/20.500.14066/4419
UL http://hdl.handle.net/20.500.14066/4419
LA eng
NO Cebrián, J., Martínez, V., Hernández, P., Krimer, D. B., Martínez-Robles, M. L., Schvartzman, J. B. and Fernández-Nestosa, M. J. (2024). Electrophoretic Mobility Assay to Separate Supercoiled, Catenated, and Knotted DNA Molecules. Bio-protocol 14(9): e4983. DOI: 10.21769/BioProtoc.4983.
NO For correspondence: María José Fernández Nestosa, mjfernandez@pol.una.py
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 02-ago-2024