RT info:eu-repo/semantics/article
T1 The diversity of sandflies (Psychodidae: Phlebotominae) and the presence of Leishmania spp. DNA in potential vectors of the Mbaracayú Forest Biosphere Reserve, Canindeyú, Paraguay : new records and findings
A1 Rolón, Miriam Soledad
A1 Rojas de Arias, Gladys Antonieta
A1 Velázquez, Myriam Celeste
A1 Britos Molinas, Milena Beatriz
A1 Arze Selich, Verónica Paola
A1 Alfonso Ruiz Díaz, Jorge Javier
A1 Salvioni Recalde, Oscar Daniel
A1 Fraenkel Cálcena, Stefanía
A1 Gómez Garay, Ana Fidelina
A1 Martínez Acosta, Nidia
A1 Vega Gómez, María Celeste
A2 Fundación Moisés Bertoni
A2 Centro para el Desarrollo de la Investigación Científica
AB Leishmaniasis is a zoonotic disease where parasites of the genus Leishmania are transmitted among hosts by sandfly vectors of the subfamily Phlebotominae. In Paraguay, studies on sandfly biodiversity are limited, even though the disease is present in its tegumentary and visceral forms. This pioneering ecoepidemiological study analyzes the diversity, abundance, and detection of Leishmania spp. DNA in sandflies from primary, secondary, and degraded forest habitats within the Mbara¬cayú Forest Biosphere Reserve. Over a year of sampling, 954 sandflies were cap¬tured, comprising 599 females and 355 males, belonging to 12 species, 10 of which are reported for the first time in Canindeyú. The species with the highest positivity for Leishmania spp. DNA were the Evandromyia cortelezzii complex (20.3%), Pintomyia monticola (18.8%), Brumptomyia brumpti (17.4%), Brumptomyia avellari (15.9%), and Psathyromyia lanei (11.6%). In contrast, Evandromyia evandroi, Micropygomyia quinquefer, Nyssomyia neivai, and Migonemyia migonei displayed rates of 4.3% or lower, while Psathyromyia shannoni complex, Brumptomyia guimaraesi, and Evandromyia termitophila were negative. The identified Leishmania species were L. amazonensis (65.2%) and L. infantum (34.8%). Additionally, dogs and their ectopar¬asites from an indigenous community were analyzed, detecting L. amazonensis DNA in 18 flea groups (54.5%) and one tick (16.7%). However, all dogs tested negative for the Rk39 test. This study highlights the risk of leishmaniasis transmission in forested and degraded ecosystems, which could have significant public health consequences, calling for deeper analyses to assess associated risks.
PB Public Library of Science
YR 2025
FD 2025-12-10
LK http://hdl.handle.net/20.500.14066/4721
UL http://hdl.handle.net/20.500.14066/4721
LA eng
NO Rolón, M. S., Rojas de Arias, A., Velázquez, M., Britos, M., Arze Selich, P., Alfonso Ruiz Díaz, J., Salvioni Recalde, O., Fraenkel Cálcena, S., Gómez Garay, A., Martínez Acosta, N., & Vega Gómez, M. C. (2025). The diversity of sandflies (Psychodidae: Phlebotominae) and the presence of Leishmania spp. DNA in potential vectors of the Mbaracayú Forest Biosphere Reserve, Canindeyú, Paraguay: new records and findings. PLoS Neglected Tropical Diseases, 19(12), Article e0013806. https://doi.org/10.1371/journal.pntd.0013806
NO Correspondencia a los autores: mcvegagomez@gmail.com (MCVG).
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 15-ago-2026