RT info:eu-repo/semantics/article T1 Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay A1 del Puerto Rodas, Ramona Florencia A1 Ozorio, Mónica A1 Trinidad, Beatriz A1 Martínez, Nidia A1 Torales, Martha Rossana A1 Franco, Luciano A1 Ferreira, Luis A1 Vera, Ninfa Isabel A2 Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud AB In Paraguay, no cases of Malaria have been recorded since 2011. Microscopy and the SnM-PCR technique were implemented to detect and characterize Plasmodium spp. both in mosquitoes and in humans residing in historically endemic areas of Paraguay, to evaluate the possibility of finding asymptomatic cases and/or Plasmodium parasites circulating in anophelines. Between 2013 and 2015, 361 human blood samples were collected on filter paper, and between 2016 and 2017, 938 female Anopheles mosquitoes were captured in 15 Paraguayan localities. All the diagnostic techniques showed negative results. We observed no asymptomatic case or Plasmodium circulating in vectors. PB Elsevier YR 2020 FD 2020-08-15 LK http://hdl.handle.net/20.500.14066/2979 UL http://hdl.handle.net/20.500.14066/2979 LA eng NO Corresponding author at: Department of Tropical Medicine, Research Institute in Health Science (IICS-UNA), National University of Asunción, Campus UNA, San Lorenzo, Paraguay. E-mail addresses: colepuerto@hotmail.com, medicinatropical@iics.una.py. (F. del Puerto). NO Consejo Nacional de Ciencia y Tecnología DS MINDS@UW RD 20-dic-2024