RT info:eu-repo/semantics/article
T1 Real-time RT-PCR for the detection and quantitation of Oropouche virus
A1 Rojas Segovia, Alejandra María
A1 Stittleburg, Victoria
A1 Cardozo Segovia, Fátima María
A1 Bopp, Nathen
A1 Cantero, César
A1 López, Sanny
A1 Bernal, Cynthia
A1 Mendoza Torres, Laura Patricia
A1 Aguilar, Patricia
A1 Pinsky, Benjamin A.
A1 Arévalo de Guillén, Yvalena
A1 Páez Acchiardi, Gloria Malvina
A1 Waggoner, Jesse J.
AB Oropouche virus (OROV) causes an acute, systemic febrile illness, and in certain regions of South America, this represents the second most common human arboviral infection after dengue virus. A new real-time RT-PCR was developed for OROV and reassortant species. The new OROV rRT-PCR proved linear across 6–7 orders of magnitude with a lower limit of 95% detection of 5.6–10.8 copies/μL. Upon testing dilutions of OROV and Iquitos virus reference genomic RNA, all dilutions with >10 copies/μL were detected in both the OROV rRT-PCR and a comparator molecular assay, but the OROV rRT-PCR detected more samples with ≤10 copies/μL (8/14 vs 0/13, respectively, P = 0.002). In a set of 100 acute-phase clinical samples from Paraguay patients with a suspected arboviral illness, no patients tested positive for OROV RNA using either assay. The OROV rRT-PCR provides a sensitive molecular assay for the study of this important yet neglected tropical arboviral infection.
PB Elsevier Inc.
SN 0732-8893
YR 2019
FD 2019-09-09
LK http://hdl.handle.net/20.500.14066/4454
UL http://hdl.handle.net/20.500.14066/4454
LA eng
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 04-nov-2024