RT info:eu-repo/semantics/article
T1 The antihypertensive and diuretic effect of crude root extract and saponins from Solanum sisymbriifolium Lam., in L-NAME-induced hypertension in rats
A1 Ibarrola Díaz, Derlis Alcides
A1 Arrúa Báez, Wilfrido Javier
A1 González Cuevas, Jazmín Estefanía
A1 Soverina Escobar, María Soledad
A1 Centurión Quintana, Juan Rafael
A1 Campuzano Benítez, Araceli Magalí
A1 Ovando Soria, Francisco Miguel
A1 Rodas González, Elena Isabel
A1 Arrúa, Karina Giselle
A1 Acevedo Barrios, María Belén
A1 Heinichen, Olga Yolanda
A1 Montalbetti, Yenny
A1 Campuzano Bublitz, Miguel Ángel
A1 Kennedy Rolón, María Luisa
A1 Figueredo Thiel, Susy Jacqueline
A1 Alvarenga Sosa, Nelson Luis
A1 Hellión Ibarrola, María del Carmen
A2 Universidad Nacional de Asunción. Facultad de Ciencias Químicas
AB Ethnopharmacological relevance: Solanum sisymbriifolium Lam., is used in Paraguayan folk medicine claiming antihypertensive and diuretic properties.Aim of the study: This study aimed to determine the influence of chronic oral administration of the crude root extract and saponins obtained from S. sisymbriifolium Lam., on the blood pressure of male and female rats with hypertension induced by L-NAME, and its consequences on diuresis, the body weight, blood glucose, and level of serum parameters of liver and kidney functionality.Materials and methods: Wistar rats were randomly divided into seven male, and seven female groups (8 animals each), which received as 6-week pretreatment, 0.9% saline solution (two groups; 0.1mL/10 g of b.w.), L-arginine (100.0 mg/kg/day), enalapril (15.0 mg/kg/day), crude extract (CESs 100.0 mg/kg/day), and saponin purified fraction (1.0, and 10.0 mg/kg/day), and treated with L-NAME (20 mg/kg/day/i.p.) twice, 1, and 6 h after pre-treatment. The animals' body weight, glycemia, and blood pressure were recorded weekly, while serum, hepatic, renal, and histological parameters were analyzed at the end of 6-week of treatment.Results: A protective effect of CESs (100.0 mg/kg/day), and saponins (1.0, and 10.0 mg/kg/day) against hypertension induced by L-NAME was verified in the systolic, diastolic, and mean blood pressure values, which were significantly lower than the positive L-NAME-hypertensive control group (male and female) at the end of the 6-week treatment. Also, pretreatment with enalapril (15.0 mg/kg/day) induced an efficient protective activity, which validates the method used. Likewise, the volume of urine, creatinine, uric acid, urea, and electrolyte excretion was enhanced at the end of 6-week of treatment in concordance with the reduction in serum level of the same parameters, compatible with the improvement of the diuretic activity. The glycemia, body weight, heart rate, and functional hepato-renal parameters were not modified after a 6-week of treatment, in comparison to the control group, indicating relatively acceptable harmless properties of CESs and saponins. Interestingly, the HDL level in females was increased in contrast to male rats by chronic saponins treatment when compared with the negative control group.Conclusions: It can be concluded that either the increment in blood pressure (systolic, diastolic, and median) or cardiorenal remodeling effects in male and female rats submitted to L-NAME-induced hypertensive condition, were prevented and well-preserved without a significant variation during a period of 6-week of pretreatment with CESs and saponins pretreatments. Likewise, an important diuretic effect was revealed after this period of treatment.
PB Elsevier B.V.
SN 0378-8741
YR 2022
FD 2022-08-13
LK http://hdl.handle.net/20.500.14066/4459
UL http://hdl.handle.net/20.500.14066/4459
LA eng
NO Ibarrola, D. A., Arrua, W., Gonzalez, J. E., Soverina Escobar, M. S., Centurión, J., Campuzano Benitez, A. M., Ovando Soria, F. M., Rodas González, E. I., Arrúa, K. G., Acevedo Barrios, M. B., Heinichen, O. Y., Montalbetti, Y., Campuzano Bublitz, M. A., Kennedy, M. L., Figueredo Thiel, S. J., Alvarenga, N. L., & Hellión Ibarrola, M. C. (2022). The antihypertensive and diuretic effect of crude root extract and saponins from Solanum sisymbriifolium Lam., in L-NAME-induced hypertension in rats. Journal of ethnopharmacology, 298, Artículo 115605. https://doi.org/10.1016/j.jep.2022.115605
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 19-dic-2024