RT info:eu-repo/semantics/article
T1 Species identification and mycotoxigenic potential of Aspergillus section Flavi isolated from maize marketed in the Metropolitan Region of Asunción, Paraguay
A1 Moura Mendes Arrua, Juliana
A1 Cazal Martínez, Cinthia Carolina
A1 Rojas Abraham, Cinthia Mabel
A1 Ferreira Benítez, Francisco Paulo
A1 Pérez Estigarribia, Pastor Enmanuel
A1 Dias, Nathalia
A1 Godoy, Patrício
A1 Costa, Jéssica
A1 Santos, Cledir
A1 Arrúa Widmer, Andrea Alejandra
A2 Cámara Paraguaya de Exportadores y Comercializadores de Cereales y Oleaginosas
AB Zea mays var. amylacea and Zea mays var. indurata are maize ecotypes from Paraguay. Aspergillus section Flavi is the main spoilage fungus of maize under storage conditions. Due to its large intraspecific genetic variability, the accurate identification of this fungal taxonomic group is difficult. In the present study, potential mycotoxigenic strains of Aspergillus section Flavi isolated from Z. mays var. indurata and Z. mays var. amylacea that are marketed in the metropolitan region of Asunción were identified by a polyphasic approach. Based on morphological characters, 211 isolates were confirmed to belong to Aspergillus section Flavi. A subset of 92 strains was identified as Aspergillus flavus by mass spectrometry MALDI-TOF and the strains were classified by MALDI-TOF MS into chemotypes based on their aflatoxins and cyclopiazonic acid production. According to the partial sequencing of ITS and CaM genes, a representative subset of 38 A. flavus strains was confirmed. Overall, 75 A. flavus strains (86%) were characterized as producers of aflatoxins. The co-occurrence of at least two mycotoxins (AF/ZEA, FUM/ZEA, and AF/ZEA/FUM) was detected for five of the Z. mays samples (63%). Considering the high mycological bioburden and mycotoxin contamination, maize marketed in the metropolitan region of Asunción constitutes a potential risk to food safety and public health and requires control measures.
PB Multidisciplinary Digital Publishing Institute
YR 2023
FD 2023-07-25
LK http://hdl.handle.net/20.500.14066/4536
UL http://hdl.handle.net/20.500.14066/4536
LA eng
NO Moura Mendes, J., Cazal Martínez, C. C., Rojas, C., Ferreira, F., Pérez Estigarribia, P., Dias, N., Godoy, P., Costa, J., Santos, C., & Arrua, A. (2023). Species identification and mycotoxigenic potential of Aspergillus section Flavi isolated from maize marketed in the Metropolitan Region of Asunción, Paraguay. Microorganisms, 11(8), Artículo 1879. https://doi.org/10.3390/microorganisms11081879
NO Correspondence: cledir.santos@ufrontera.cl (C.S.); andrea.arrua@cemit.una.py (A.A.)
NO This article belongs to the Special Issue Mass Spectrometry: An Undeniable Tool in Current Microbiology 2.0.
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 12-mar-2025