RT info:eu-repo/semantics/article
T1 Screening of natural products inhibitors of SARS-CoV-2 entry
A1 González Maldonado, Pamela
A1 Alvarenga Sosa, Nelson Luis
A1 Burgos Edwards, Alberto Javier
A1 Flores Giubi, María Eugenia
A1 Barúa Chamorro, Javier Enrique
A1 Romero Rodríguez, María Cristina
A1 Soto Rifo, Ricardo Andrés
A1 Valiente Echeverría, Fernando Andrés
A1 Langjahr Penayo, Patricia Elena
A1 Cantero González, Guadalupe Luján
A1 Sotelo Torres, Pablo Hernán
A2 Fundación Facultad de Ciencias Químicas
AB The COVID-19 pandemic has led to the search for new molecules with antiviral activity against SARS-CoV-2. The entry of the virus into the cell is one of the main targets for inhibiting SARS-CoV-2 infection. Natural products are an important source of new therapeutic alternatives against diseases. Pseudotyped viruses allow the study of SARS-CoV-2 viral entry inhibitors, and due to their simplicity, they allow the screening of a large number of antiviral candidates in Biosafety Level 2 facilities. We used pseudotyped HIV-1 with the D614G SARS-CoV-2 spike glycoprotein to test its ability to infect ACE2-expressing HEK 293T cells in the presence of diverse natural products, including 21 plant extracts, 7 essential oils, and 13 compounds from plants and fungi. The 50% cytotoxic concentration (CC50) was evaluated using the resazurin method. From these analyses, we determined the inhibitory activity of the extract of Stachytarpheta cayennensis, which had a halfmaximal inhibitory concentration (IC50) of 91.65 _g/mL, a CC50 of 693.5 _g/mL, and a selectivity index (SI) of 7.57, indicating its potential use as an inhibitor of SARS-CoV-2 entry. Moreover, our work indicates the usefulness of the pseudotyped-virus system in the screening of SARS-CoV-2 entry inhibitors.
PB Multidisciplinary Digital Publishing Institute
YR 2022
FD 2022-03-07
LK http://hdl.handle.net/20.500.14066/4591
UL http://hdl.handle.net/20.500.14066/4591
LA eng
NO González-Maldonado, P., Alvarenga, N., Burgos-Edwards, A., Flores-Giubi, M. E., Barúa, J. E., Romero-Rodríguez, M. C., Soto-Rifo, R., Valiente-Echeverría, F., Langjahr, P., Cantero-González, G., & Sotelo, P. H. (2022). Screening of natural products inhibitors of SARS-CoV-2 entry. Molecules, 27(5), Article 1743. https://doi.org/10.3390/molecules27051743
NO Correspondence: phsotelo@qui.una.py.
NO This article belongs to the Special Issue Improvements and Opportunities on Natural Products for Novel Drug Discovery.
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 03-sep-2026