RT info:eu-repo/semantics/article
T1 Synergism of in vitro plasmodicidal activity of phospholipase A2 isoforms isolated from panamanian Bothrops asper venom
A1 Simões Silva, Rodrigo
A1 Alfonso Ruiz Díaz, Jorge Javier
A1 Gómez Garay, Ana Fidelina
A1 Sobrinho, Juliana C.
A1 Kayano, Anderson M.
A1 de Medeiros, Daniel S.S.
A1 Teles, Carolina B.G.
A1 Quintero, Arístides
A1 Fuly, André L.
A1 Vega Gómez, María Celeste
A1 Pereira, Soraya S.
A1 da Silva, Saulo L.
A1 Stábeli, Rodrigo G.
A1 Soares, Andreimar M.
AB Bothrops asper is one of the most important snake species in Central America, mainly because of its medical importance in countries like Ecuador, Panama and Costa Rica, where this species causes a high number of snakebite accidents. Several basic phospholipases A2 (PLA2s) have been previously characterized from B. asper venom, but few studies have been carried out with its acidic isoforms. In addition, since snake venom is a rich source of bioactive substances, it is necessary to investigate the biotechnological potential of its components. In this context, this study aimed to carry out the biochemical characterization of PLA2 isoforms isolated from B. asper venom and to evaluate the antiparasitic potential of these toxins. The venom and key fractions were subjected to different chromatographic steps, obtaining nine PLA2s, four acidic ones (BaspAc-I, BaspAc-II, BaspAc-III and BaspAc-IV) and five basic ones (BaspB-I, BaspB-II, BaspB-III, BaspB-IV and BaspB-V). The isoelectric points of the acidic PLA2s were also determined, which presented values ranging between 4.5 and 5. The findings indicated the isolation of five unpublished isoforms, four Asp49-PLA, corresponding to the group of acidic isoforms, and one Lys49-PLA2-like. Acidic PLA2s catalyzed the degradation of all substrates evaluated; however, for the basic PLA2s, there was a preference for phosphatidylglycerol and phosphatidic acid. The antiparasitic potential of the toxins was evaluated, and the acidic PLA2s demonstrated action against the epimastigote forms of T. cruzi and promastigote forms of L. infantum, while the basic PLA2s BaspB-II and BaspB-IV showed activity against P. falciparum. The results indicated an increase of up to 10 times in antiplasmodial activity, when the Asp49-PLA2 and Lys49-PLA2 were associated with one another, denoting synergistic action between these PLA2 isoforms. These findings correspond to the first report of synergistic antiplasmodial action for svPLA2s, demonstrating that these molecules may be important targets in the search for new antiparasitic agents.
PB Elsevier
SN 0009-2797
YR 2021
FD 2021-07-21
LK http://hdl.handle.net/20.500.14066/4592
UL http://hdl.handle.net/20.500.14066/4592
LA eng
NO Simões-Silva, R., Alfonso, J. J., Gómez, A. F., Sobrinho, J. C., Kayano, A. M., de Medeiros, D. S. S., Teles, C. B. G., Quintero, A., Fuly, A. L., Vega Gómez, C., Pereira, S. S., da Silva, S. L., Stábeli, R. G., & Soares, A. M. (2021). Synergism of in vitro plasmodicidal activity of phospholipase A2 isoforms isolated from panamanian Bothrops asper venom. Chemico-Biological Interactions, 346, Article 109581.https://doi.org/10.1016/j.cbi.2021.109581
NO Corresponding author. Fiocruz Rondonia, Rua da Beira, 7176, Bairro Lagoa, 76812, Porto Velho, Rondonia, Brazil. E-mail address: andreimar.soares@fiocruz.br (A.M. Soares).
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 03-sep-2026