RT info:eu-repo/semantics/article
T1 Genomic surveillance of invasive Neisseria meningitidis isolates circulating in Paraguay, 2009-2021
A1 León Ayala, María Eugenia
A1 Kawabata Inoue, Aníbal Yasunari
A1 Nagai Oji, Cristina Minako
A1 Rojas Perdomo, Liliana María
A1 Chamorro Cortesi, Gustavo Adolfo
A1 Weiler Gustafson, Natalie
A1 Orrego Miranda, María Verónica
A1 Martínez Mendoza, Lucía Jazmín
A1 Silva de Lemos, Ana Paula
A1 Camargo, Carlos Henrique
A1 López, Evelyn
A1 Irala, Juan
A1 Blasco, Raquel
A1 Gómez, Gloria
A1 Ortellado Canese, Juana Medarda
A1 Leguizamón, Myrian
A1 Ortiz, Rosana
A2 Centro de Estudios y Formación para el Ecodesarrollo - ALTER VIDA
AB Introduction. Invasive meningococcal disease (IMD) remains a significant public health concern and one of the leading causes of morbidity and mortality worldwide. In Paraguay, IMD continues to pose a serious health challenge.Methodology. A retrospective, descriptive, cross-sectional observational study was conducted. Whole-genome sequencing was employed to perform multilocus sequence sequence typing (MLST), vaccine antigens characterization, and antimicrobial resistance gene profiling of Neisseria meningitidis isolates obtained from children and adults with IMD, as part of the Bacterial Meningitis Surveillance Program in Paraguay during 2009–2021.Results. Eight clonal complexes were identified, with cc103 being the most frequent, followed by cc11, cc35, cc167, cc41/44, cc865, cc32, and cc60. Regarding vaccine antigens, the fHbp variant A05 peptide targeted by MenB-FHbp was absent, whereas the B01 variant was detected in 3 out of 70 isolates (4.3%). For the 4CMenB vaccine components, peptide 1 of fHbp (subfamily B/variant 1), NHBA peptide 2, and NadA peptide 8 were absent, while PorA P1.7–2,4 was found in one isolate (1.4%). Mutated penA alleles were present in 73.7% of the isolates. Major peptides (PorA, PorB, FetA, NHBA, fHbp) were correlated with clonal complexes and serogroups.Conclusion. This is the first genomic study of Neisseria meningitidis conducted in Paraguay, enabling a novel understanding of the genetic characteristics of invasive isolates. The generation of genomic data is essential for monitoring the spread of circulating lineages or clones that may alter their antigenic profiles to evade host immune responses.
PB Springer Nature
SN 1139-6709
YR 2025
FD 2025-06-17
LK http://hdl.handle.net/20.500.14066/4597
UL http://hdl.handle.net/20.500.14066/4597
LA eng
NO León, M. E., Kawabata, A. Y., Nagai, C. M., Rojas, L. M., Chamorro, G., Weiler, N., Orrego, M. V., Martínez, L. J., Silva de Lemos, A. P., Camargo, C. H., López, E., Irala, J., Blasco, R., Gómez, G., Ortellado, J., Leguizamón, M., & Ortiz, R. (2025). Genomic surveillance of invasive Neisseria meningitidis isolates circulating in Paraguay, 2009-2021. International Microbiology.https://doi.org/10.1007/s10123-025-00685-0
NO Corresponding author. Correspondence to Aníbal Yasunari Kawabata; bhatayazz2204@hotmail.com.
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 04-sep-2026