RT info:eu-repo/semantics/article
T1 Implementation of a multiplex rRT-PCR for Zika, Chikungunya, and Dengue viruses: improving arboviral detection in an endemic region
A1 Cantero Ramírez, César Andrés
A1 Cardozo Segovia, Fátima María
A1 Waggoner, Jesse J.
A1 Pinsky, Benjamin A.
A1 Espínola Cano, Aníbal Francisco
A1 Infanzón Ramos, María Belén
A1 Acosta de Hetter, María Eugenia
A1 Aria Zaya, Laura Silvana
A1 Arévalo de Guillén, Ivalena
A1 Teresa, Cuevas
A1 Vicenta, Rojas
A1 Segovia, Clotilde
A1 Centurión, Ana
A1 Rojas Segovia, Alejandra María
AB Arboviral diagnosis has been complicated throughout the tropical and subtropical Americas by the recent co-circulation of Zika virus (ZIKV), chikungunya virus (CHIKV), and dengue virus (DENV). The aim of this study was to implement a multiplex real-time RT-PCR (rRT-PCR) for ZIKV, CHIKV, and DENV in Paraguay to test patients who were clinically suspected of having dengue.Wetested 110 sera from patients who presented to the Hospital de Cl´ınicas in 2016 and had testing for DENV nonstructural protein 1 (NS1; 40 positive and 70 negative). Using a composite reference standard, we confirmed 51 dengue cases (46.4%): 38/40 NS1 positive and 13/70 NS1 negative. Chikungunya virus and ZIKV were detected in one sample each, both wereDENVNS1negative. TheNS1test demonstrated good agreement with rRT-PCR for DENV. However, multiplex rRT-PCR identified a subset of dengue cases and additional arboviral infections that would not be detected if NS1 assays are relied upon for diagnosis.
PB American Society of Tropical Medicine and Hygiene
SN 0002-9637
YR 2020
FD 2020-01-13
LK http://hdl.handle.net/20.500.14066/4603
UL http://hdl.handle.net/20.500.14066/4603
LA eng
NO Cantero, C., Cardozo, F., Waggoner, J. J., Pinsky, B. A., Espínola, A., Infanzón, B., Acosta, M. E., Aria, L., Arévalo de Guillén, Y., Cuevas, T., Rojas, V., Segovia, C., Centurión, A., & Rojas, A. (2020). Implementation of a multiplex rRT-PCR for Zika, Chikungunya, and Dengue viruses: improving arboviral detection in an endemic región. The American Journal of Tropical Medicine and Hygiene, 102(3), 625-628.https://doi.org/10.4269/ajtmh.19-0707
NO Address correspondence to Alejandra Rojas, Departamento de Producción, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Dr. Cecilio Báez y Dr. Villamayor Campus Universitario, San Lorenzo 2169, Asunción, Paraguay. E-mail: arojass@iics.una.py.
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 03-sep-2026