RT info:eu-repo/semantics/article
T1 Antiviral activity of two Acanthospermum species against herpes simplex virus 1
A1 Cantero González, Guadalupe Luján
A1 Alvarenga Sosa, Nelson Luis
A1 Florentín Pavía, Marcos Marcelo
A1 González Maldonado, Pamela
A1 Sotelo Torres, Pablo Hernán
A2 Universidad Nacional de Asunción. Facultad de Ciencias Químicas
A2 Fundación Facultad de Ciencias Químicas
AB Ethnopharmacological relevance. Acanthospermum species are used in traditional medicine for treating various pathologies, including bacterial and viral infections. In a screening study, we identified the activity of the ethanolic extracts of Acanthospermum australe and Acanthospermum hispidum against herpes simplex virus 1 (HSV-1).Aim of the study. In this work, we analyzed the phytochemical profile and antiviral activity of the chemical fractionation products of Acanthospermum australe and Acanthospermum hispidum. Additionally, we identified the effect of these fractions on different steps of the viral cycle.Materials and methods. Acanthospermum samples were extracted with methanol and further partitioned with solvents of increasing polarities: hexane, chloroform, ethyl acetate, and butanol. Cytotoxicity and antiviral activity were analyzed for each fraction. The active fractions were tested to identify the virucidal effect and the inhibition of virus–cell binding. Further, the effect of these fractions on the replication and viral gene was quantitated by qPCR, and the expression of gD protein was evaluated by Western blot.Results. The chloroform and hexane fractions of Acanthospermum hispidum and Acanthospermum australe showed dose-dependent antiviral activity. The chloroform fraction inhibited the virus–cell binding and virus cycle in a post-entry mechanism by decreasing replication and the expression of early and late viral genes. The hexane fraction did not inhibit virus binding; however, it showed antiviral activity in post-entry events by inhibiting the immediate-early, early, and late genes. We identified in both species the presence of 3.6-dimetoxiapigenin, axillarin, and penduletin in the chloroform fraction and methyl-(Z,Z)-9,12-octadecadienoate and phytol in the hexane fraction.Conclusions. Acanthospermum hispidum and Acanthospermum australe possess antiviral activity against HSV-1 and affect different steps of the viral cycle. These characteristics make them good candidates for developing phytotherapeutic products against HSV-1.
PB Elsevier
SN 0378-8741
YR 2022
FD 2022-12-05
LK http://hdl.handle.net/20.500.14066/4639
UL http://hdl.handle.net/20.500.14066/4639
LA eng
NO Cantero-González, G., Alvarenga, N., Florentín-Pavía, M. M., González-Maldonado, P., & Sotelo, P. H. (2022). Antiviral activity of two Acanthospermum species against herpes simplex virus 1. Journal of Ethnopharmacology, 303, Article 115958.https://doi.org/10.1016/j.jep.2022.115958
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 03-sep-2026