RT info:eu-repo/semantics/article
T1 Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay
A1 Rodríguez Valinotti, María Fátima
A1 Nara Pereira, Eva Megumi
A1 Martínez Pereira, Iara Magaly
A1 Rodríguez Valinotti, Rosmary
A1 Rodríguez Sánchez, Antonio
A2 Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud
K1 Cría de ganado
K1 Flaviviridae
K1 Ganado bovino
K1 PCR
K1 Pestivirus
K1 Pestivirus de la diarrea bovina
K1 Livestock-raising
K1 Flaviviridae
K1 Cattle
K1 PCR
K1 Pestivirus
K1 Bovine diarrhoea pestivirus
AB Bovine viral diarrhea virus (BVDV) is the most prevalent pathogen in cattle and causes significant economic losses due to its severe clinical manifestations. It belongs to the family Flaviviridae and is distributed in species A, B, and H within the genus Pestivirus. The objective of this study was to detect and characterize BVDV using molecular techniques (RT-PCR) in semen and aborted fetuses samples that were sent to the CEDIVEP (Veterinary Diagnostic Center of Paraguay) laboratory. Seventy-three samples of semen from bulls were analyzed, and 54.7% of the samples were positive for Pestivirus A. The presence of Pestivirus A and H was detected in 2/8 spontaneously aborted fetuses. The genotypes of four individual samples of type A and four samples of type H organs were confirmed by partial sequencing of the 5-UTR region. The presence of BVDV was confirmed by molecular techniques for the first time in our country through its detection in different types of samples, as well as the presence of two genotypes. This suggests that the circulation of this virus can cause significant losses in cattle production in Paraguay.
PB Multidisciplinary Digital Publishing Institute
YR 2025
FD 2025-09-24
LK http://hdl.handle.net/20.500.14066/4663
UL http://hdl.handle.net/20.500.14066/4663
LA eng
NO Rodriguez Valinotti, M. F., Nara Pereira, E. M., Martinez Pereira, M., Rodriguez Valinotti, R., & Rodriguez Sanchez, A. (2025). Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay. Viruses, 17(10), Article 1295. https://doi.org/10.3390/v17101295
NO Correspondence: m.rodriguez@cedivep.com.py; Tel.: +595-984179-025 (ext. 111422).
NO This article belongs to the Special Issue Bovine Viral Diarrhea Viruses and Other Pestiviruses.
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 03-sep-2026