RT info:eu-repo/semantics/article
T1 Evaluation of antioxidant and anthelmintic properties of Tithonia diversifolia extracts against gastrointestinal nematode eggs using in vitro assays
A1 Meza Ocampos, Griselda Asunción
A1 Custodio, Luisa
A1 Mereles Ceuppens, Laura Graciela
A1 Ávila, Rocío
A1 Villalba Fariña, Domitila
A1 Llano Obregón, Silvio Ismael
A1 Sánchez Madsen, Fátima Belén
AB Parasitic diseases caused by gastrointestinal nematodes (GIN) are considered important limiting factors for livestock production worldwide, as they significantly compromise animal welfare and increase production costs. For years, parasite control has relied exclusively on the use of synthetic drugs. Resistance of GIN to commercial anthelmintics has been increasingly reported throughout the world. Therefore, alternative approaches have emerged for controlling GIN in farm animals, including the exploration of plants with bioactive potential. Tithonia diversifolia can be harvested year-round, and all parts of the plant have been used in folk medicine for a wide range of diseases. This study evaluated the in vitro antioxidant properties of water and 70% aqueous acetone extracts of T. diversifolia aerial parts, including leaves, branches, and flowers, using radical-based methods, and assessed its anthelmintic potential through a parasite model, employing the egg hatching assay (EHA) with water extracts. Additionally, the total polyphenolic content of the extracts was determined. These findings highlight T. diversifolia as a promising natural source of antioxidant and anthelmintic compounds.
PB MyJoVE Corporation
YR 2025
FD 2025-08-01
LK http://hdl.handle.net/20.500.14066/4667
UL http://hdl.handle.net/20.500.14066/4667
LA eng
NO Meza Ocampos, G. A., Custodio, L., Mereles, L., Ávila, R., Villalba, D., Llano Obregon, I., & Sánchez Madsen, F. (2025). Evaluation of antioxidant and anthelmintic properties of Tithonia diversifolia extracts against gastrointestinal nematode eggs using in vitro assays. Journal of Visualized Experiments, Article e67760.https://doi.org/10.3791/67760
NO Correspondence: mezagriselda@hotmail.com.
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 03-sep-2026