RT info:eu-repo/semantics/article
T1 Secretome analysis of the phytopathogen Macrophomina phaseolina cultivated in liquid medium supplemented with and without soybean leaf infusion
A1 Pineda Fretez, Amiliana Beatriz
A1 Orrego Durañona, Amelia Adriana
A1 Iehisa Ouchi, Julio César Masaru
A1 Flores Giubi, María Eugenia
A1 Barúa Chamorro, Javier Enrique
A1 Sánchez Lucas, Rosa
A1 Jorrín Novo, Jesús
A1 Romero Rodríguez, María Cristina
A2 Fundación Facultad de Ciencias Químicas
AB Macrophomina phaseolina (Tassi) Goid. is a fungal pathogen that causes root and stem rot in several economically important crops. However, most of disease control strategies have shown limited effectiveness. Despite its impact on agriculture, molecular mechanisms involved in the interaction with host plant remains poorly understood. Nevertheless, it has been proven that fungal pathogens secrete a variety of proteins and metabolites to successfully infect their host plants. In this study, a proteomic analysis of proteins secreted by M. phaseolina in culture media supplemented with soybean leaf infusion was performed. A total of 250 proteins were identified with a predominance of hydrolytic enzymes. Plant cell wall degrading enzymes together peptidases were found, probably involved in the infection process. Predicted effector proteins were also found that could induce plant cell death or suppress plant immune response. Some of the putative effectors presented similarities to known fungal virulence factors. Expression analysis of ten selected protein-coding genes showed that these genes are induced during host tissue infection and suggested their participation in the infection process. The identification of secreted proteins of M. phaseolina could be used to improve the understanding of the biology and pathogenesis of this fungus. Although leaf infusion was able to induce changes at the proteome level, it is necessary to study the changes induced under conditions that mimic the natural infection process of the soil-borne pathogen M. phaseolina to identify virulence factors.
PB Elsevier
SN 1878-6146
YR 2023
FD 2023-04-10
LK http://hdl.handle.net/20.500.14066/4722
UL http://hdl.handle.net/20.500.14066/4722
LA eng
NO Corresponding authors: jcmiehisa@qui.una.py; rromero@qui.una.py.
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 04-sep-2026