RT info:eu-repo/semantics/article T1 Characterization of Trichoderma species from agricultural soils of Paraguay T2 Caracterización de especies de Trichoderma de suelos agrícolas de Paraguay A1 Sanabria Velázquez, Andrés Dejesús A1 Florentín Pavía, Marcos Marcelo A1 Insaurralde Ayala, Lorena A1 Flores Giubi, María Eugenia A1 Romero Rodríguez, María Cristina A1 Sotelo Torres, Pablo Hernán A1 Barúa Chamorro, Javier Enrique A2 Universidad Nacional de Asunción. Facultad de Ciencias Químicas AB There is a growing interest in the development of sustainable alternatives to the use of chemical pesticides for pest management in agricultural systems. This research aimed to isolate and characterize native strains of Trichoderma spp. from different soils of Paraguay using morphological and molecular criteria. We processed plant and soil samples from eight commercial farms distributed in different departments of Paraguay and isolated 14 monosporic isolates of Trichoderma spp., obtaining two isolates from the Department of Alto Paraná (FCQ36 and FCQ37), four isolates from Cordillera (FCQ42, FCQ43, FCQ44, and FCQ46), one isolate from Central (FCQ32), and seven isolates from Itapúa (FCQ13, FCQ16, FCQ18, FCQ19, FCQ21, FCQ23, and FCQ47). In addition, phylogenetic analyses using the ITS and tef1α loci were carried out. A better resolution of the tef1a gene than the ITS region was observed. Moreover, a third phylogenetic tree from the concatenated ITS and tef1α sequences matrix was generated, obtaining the same topology with higher bootstrap support values. Through this approach, we reported for the first time the presence of Trichoderma koningiopsis (FCQ19, FCQ36, and FCQ37), Trichoderma neo¬koningii (FCQ13), and Trichoderma asperellum (FCQ42, FCQ43, FCQ44, and FCQ46), Trichoderma brevicompactum (FCQ18 and FCQ21), and Trichoderma longibrachiatum (FCQ 47) in Paraguay. The Trichoderma species identified in this study can be used to develop effective biocontrol products for agricultural and industrial purposes in Paraguay. PB Universidad Nacional de Colombia. Facultad de Ciencias Agrarias. Centro Editorial YR 2023 FD 2023-12-31 LK http://hdl.handle.net/20.500.14066/4724 UL http://hdl.handle.net/20.500.14066/4724 LA eng NO Sanabria-Velázquez, A. D., Florentín Pavía, M. M., Insaurralde Ayala, L., Flores-Giubi, M. E., Romero-Rodríguez, M. C., Sotelo, P. H. & Barúa, J. E. (2023). Characterization of Trichoderma species from agricultural soils of Paraguay. Agronomía Colombiana, 41(3), Artículo e111299. https://doi.org/10.15446/agron.colomb.v41n3.111299 NO Corresponding author: javierbarua@qui.una.py. NO Consejo Nacional de Ciencia y Tecnología DS MINDS@UW RD 04-sep-2026