RT info:eu-repo/semantics/article
T1 Enzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay
A1 Resquín Romero, Gloria Arminda
A1 Silva Mattos, Vanessa
A1 da Mata dos Santos Monteiro, Jessica
A1 López Nicora, Horacio Daniel
A1 Amarilla, Shyrley Paola
A1 Chamorro Díaz, Sergio Manuel
A1 Moral Moral, Juan
A1 Dechechi Gomes Carneiro, Regina María
AB Tomato is a major crop in Paraguay, where it provides a source of employment and income for households. Tomato production can be affected by root-knot nematodes, especially Meloidogyne spp. The unequivocal identification of Meloidogyne spp. in Paraguay has not been conducted yet. This study aims to identify Meloidogyne species in eight tomato production districts of this country by biochemical and molecular techniques. Females of Meloidogyne spp. were extracted from tomato roots and characterized using esterase isozyme phenotypes. In addition, DNA was extracted from nematode eggs, and species-specific SCARs (sequence-characterized amplified regions) were used to confirm the diagnosis. Nematodes were detected in 100% of studied samples (prevalence), of which M. incognita (Est: I2, Rm: 1.1;1.2) and M. javanica (Est: J3, Rm: 1.0, 1.20, 1.35) were present in 39.13% and 26.08% of samples, respectively. One population (8.69%) of Meloidogyne sp. presenting an atypical esterase profile (Rm: 1.0 and 1.3) was only detected in Julián Augusto Saldívar District. Mixed populations, mostly M. incognita and M. javanica, were observed in 26.08% of samples. The SCAR primers incK14F/incK14R amplified specific fragments for M. incognita (399 bp) and M. javanica (670 bp), confirming the enzymatic results. Here, we present the first study of root-knot nematode identification at the species level in Paraguay.
PB Multidisciplinary Digital Publishing Institute
SN 2073-4395
YR 2023
FD 2023-02-25
LK http://hdl.handle.net/20.500.14066/4726
UL http://hdl.handle.net/20.500.14066/4726
LA eng
NO Resquín-Romero, G., Mattos, V. S., Monteiro, J. M. S., Lopez-Nicora, H. D., Amarilla, S. P., Chamorro-Diaz, S., Moral, J., & Carneiro, R. M. D. G. (2023). Enzymatic and Molecular Identification of Meloidogyne Species in Tomato Orchards in Paraguay. Agronomy, 13(3), Article 670. https://doi.org/10.3390/agronomy13030670
NO Correspondence: gloresqx@agr.una.py (G.R.-R.); regina.carneiro@embrapa.br (R.M.D.G.C.); Tel.: +595-21-585606/10 (G.R.-R.); +55-(61)-3448-4433 (R.M.D.G.C.); Fax: +595-21-585613 (G.R.-R.); +55-(61)-3448-4890 or +55-(61)-3448-4891 (R.M.D.G.C.).
NO This article belongs to the Section Pest and Disease Management.
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 03-sep-2026