RT info:eu-repo/semantics/article
T1 Terpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion
A1 Salinas Dávalos, Claudia de los Ángeles
A1 Florentín Candia, Gladys Melisa
A1 Rodríguez Acosta, Fátima
A1 Alvarenga Sosa, Nelson Luis
A1 Guillén Fretes, Rosa María
AB The biofilm is a conglomerate of cells surrounded by an extracellular matrix, which contributes to the persistence of infections. The difficulty in removing the biofilm drives the research for new therapeutic options. In this work, the effect of terpenes (−)-trans-Caryophyllene, (S)-cis-Verbenol, (S)-(−)-Limonene, (R)-(+)-Limonene, and Linalool was evaluated, individually and in combinations on bacterial growth, by assay with resazurin; the formation of biofilm, by assay with violet crystal; and the expression of associated genes, by real-time PCR, in two clinical isolates of Staphyloccocus aureus, ST30-t019 and ST5-t311, responsible for more than 90% of pediatric infections by this pathogen in Paraguay. All combinations of terpenes can inhibit biofilm formation in more than 50% without affecting bacterial growth. The most effective combination was (−)-trans-Caryophyllene and Linalool at a 500 μg/mL concentration for each, with an inhibition percentage of 88%. This combination decreased the expression levels of the sdrD, spa, agr, and hld genes associated with the initial cell adhesion stage and quorum sensing. At the same time, it increased the expression levels of the cap5B and cap5C genes related to the production of capsular polysaccharides. The combinations of compounds tested are promising alternatives to inhibit biofilm formation in S. aureus.
PB Multidisciplinary Digital Publishing Institute
SN 2076-2607
YR 2022
FD 2022-07-28
LK http://hdl.handle.net/20.500.14066/4740
UL http://hdl.handle.net/20.500.14066/4740
LA eng
NO Salinas, C., Florentín, G., Rodríguez, F., Alvarenga, N., & Guillén, R. (2022). Terpenes Combinations Inhibit Biofilm Formation in Staphyloccocus aureus by Interfering with Initial Adhesion. Microorganisms, 10(8), 1527. https://doi.org/10.3390/microorganisms10081527
NO Correspondence: nelson@qui.una.py (N.A.); rguillen@qui.una.py (R.G.); Tel.: +595-981446181 (N.A.); +595-994318520 (R.G.).
NO This article belongs to the Section Biofilm.
NO Consejo Nacional de Ciencia y Tecnología
DS MINDS@UW
RD 03-sep-2026