RT Conference Proceedings
T1 Changes in the Topology of DNA Replication Intermediates: In vivo vs In vitro
A1 Schvartzman, Jorge Bernardo
A1 Martínez, Víctor
A1 Hernández, Pablo
A1 Krimer, Dora B
A1 Fernández Nestosa, María José
A2 Universidad Nacional de Asunción - Facultad Politécnica
AB Most of the methods used to analyze DNA, including electrophoresis, electron microscopy or atomic force microscopy, involve de-proteinization, and it is well known that the removal of proteins affects DNA topology. After de-proteinization in vitro, the topology of replication intermediates changes significantly. A comprehensive analysis of the topological changes introduced during DNA isolation (de-proteinization) is important to get a better understanding of DNA topology in vivo. The topology of replication intermediates examined by electrophoresis, electron microscopy or atomic force microscopy in vitro does not necessarily represent the situation in vivo.
YR 2021
FD 2021
LK http://hdl.handle.net/20.500.14066/3307
UL http://hdl.handle.net/20.500.14066/3307
LA eng
NO CONACYT - Consejo Nacional de Ciencias y Tecnología
DS MINDS@UW
RD 06-jun-2024