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dc.contributor.authorBorges Strauss, Adolfo 
dc.contributor.authorRojas de Arias, Gladys Antonieta 
dc.contributor.authorde Almeida Lima, Sabrina
dc.contributor.authorLomonte, Bruno
dc.contributor.authorDíaz, Cecilia
dc.contributor.authorChávez Olórtegui, Carlos
dc.contributor.authorGraham, Matthew R.
dc.contributor.authorKalapothakis, Evanguedes
dc.contributor.authorCoronel, Cathia Cecilia 
dc.contributor.authorde Roodt, Adolfo R.
dc.contributor.otherCentro para el Desarrollo de la Investigación Científicaes
dc.date.accessioned2024-03-30T23:41:02Z
dc.date.available2024-03-30T23:41:02Z
dc.date.issued2020-12-14
dc.identifier.citationBorges, A., Rojas de Arias, A., de Almeida Lima, S., Lomonte, B., Díaz, C., Chávez-Olórtegui, C., Graham, M. R., Kalapothakis, E., Coronel, C., & de Roodt, A. R. (2020). Genetic and toxinological divergence among populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones: Buthidae) inhabiting Paraguay and Argentina. PLOS Neglected Tropical Diseases, 14(12), Artículo e0008899. https://doi.org/10.1371/journal.pntd.0008899en
dc.identifier.issn1935-2727es
dc.identifier.otherhttps://doi.org/10.1371/journal.pntd.0008899
dc.identifier.urihttp://hdl.handle.net/20.500.14066/4400
dc.descriptionAutor de correspondencia: borges.adolfo@gmail.comes
dc.description.abstractEnvenoming by scorpions in genus Tityus is a public health problem in Tropical America. One of the most medically significant species is Tityus trivittatus, which is known to occur from southwest Brazil to central-northern and eastern Argentina. In this work, we studied the lethality, composition, antigenicity, and enzymatic activity of venom from a T. trivittatus population found further north in urban areas of eastern Paraguay, where it has caused serious envenomation of children. Our results indicate that the population is of medical importance as it produces a potently toxic venom with an LD50 around 1.19 mg/kg. Venom neutralization in preliminary mouse bioassays was complete when using Brazilian anti-T. serrulatus antivenom but only partial when using Argentinean anti-T. trivittatus antivenom. Venom competitive solid-phase enzyme immunoassays and immunoblotting from Argentinean and Paraguayan T. trivittatus populations indicated that antigenic differences exist across the species range. SDS-PAGE showed variations in type and relative amounts of venom proteins between T. trivitattus samples from Argentina and Paraguay. MALDI-TOF mass spectrometry indicated that while some sodium channel toxins are shared, including β-toxin Tt1g, others are population-specific. Proteolytic activity by zymography and peptide identification through nESI-MS/MS also point out that population-specific proteases may exist in T. trivitattus, which are postulated to be involved in the envenoming process. A time-calibrated molecular phylogeny of mitochondrial COI sequences revealed a significant (8.14%) genetic differentiation between the Argentinean and Paraguayan populations, which appeared to have diverged between the mid Miocene and early Pliocene. Altogether, toxinological and genetic evidence indicate that T. trivitattus populations from Paraguay and Argentina correspond to distinct, unique cryptic species, and suggest that further venom and taxonomic diversity exists in synanthropic southern South American Tityus than previously thought.es
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnologíaes
dc.format.extent25 páginases
dc.language.isoenges
dc.publisherPublic Library of Scienceen
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.otherAntivenenos/farmacologíaes
dc.subject.otherEscorpioneses
dc.subject.otherEscorpiones/fisiologíaes
dc.subject.otherEscorpiones/genéticaes
dc.subject.otherFilogeniaes
dc.subject.otherPonzoñases
dc.subject.otherVenenos de escorpión/metabolismoes
dc.subject.otherVenenos de escorpión/químicaes
dc.subject.otherVenenos de escorpión/toxicidades
dc.subject.otherAntivenins/pharmacologyes
dc.subject.otherScorpionses
dc.subject.otherScorpions/physiologyes
dc.subject.otherScorpions/geneticses
dc.subject.otherPhylogenyes
dc.subject.otherVenomses
dc.subject.otherScorpion venoms/metabolismes
dc.subject.otherScorpion venoms/chemistryes
dc.subject.otherScorpion venoms / toxicityes
dc.titleGenetic and toxinological divergence among populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones: Buthidae) inhabiting Paraguay and Argentinaes
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1371/journal.pntd.0008899es
dc.description.fundingtextPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Programa de repatriación y radicación de investigadores del exteriores
dc.description.fundingtextPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Programa Nacional de Incentivo a los Investigadoreses
dc.identifier.essn1935-2735es
dc.issue.number12es
dc.journal.titlePLOS Neglected Tropical Diseasesen
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.copyright© 2020 Borges et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.es
dc.volume.number14es


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