Implementation of a multiplex rRT-PCR for Zika, Chikungunya, and Dengue viruses: improving arboviral detection in an endemic region

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URI
http://hdl.handle.net/20.500.14066/4603
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Author(s)
Cantero Ramírez, César AndrésCONACYT Authority; Cardozo Segovia, Fátima MaríaCONACYT Authority; Waggoner, Jesse J.; Pinsky, Benjamin A.; Espínola Cano, Aníbal Francisco; Infanzón Ramos, María BelénCONACYT Authority; Acosta de Hetter, María EugeniaCONACYT Authority; Aria Zaya, Laura SilvanaCONACYT Authority; Arévalo de Guillén, IvalenaCONACYT Authority; Teresa, Cuevas; Vicenta, Rojas; Segovia, Clotilde; Centurión, Ana; Rojas Segovia, Alejandra MaríaCONACYT Authority
Date of publishing
2020-01-13
Type of publication
info:eu-repo/semantics/article
Subject(s)
Abstract
Arboviral diagnosis has been complicated throughout the tropical and subtropical Americas by the recent co-circulation of Zika virus (ZIKV), chikungunya virus (CHIKV), and dengue virus (DENV). The aim of this study was to implement a multiplex real-time RT-PCR (rRT-PCR) for ZIKV, CHIKV, and DENV in Paraguay to test patients who were clinically suspected of having dengue.Wetested 110 sera from patients who presented to the Hospital de Cl´ınicas in 2016 and had testing for DENV nonstructural protein 1 (NS1; 40 positive and 70 negative). Using a composite reference standard, we confirmed 51 dengue cases (46.4%): 38/40 NS1 positive and 13/70 NS1 negative. Chikungunya virus and ZIKV were detected in one sample each, both wereDENVNS1negative. TheNS1test demonstrated good agreement with rRT-PCR for DENV. However, multiplex rRT-PCR identified a subset of dengue cases and additional arboviral infections that would not be detected if NS1 assays are relied upon for diagnosis.
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