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Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay
dc.contributor.author | del Puerto Rodas, Ramona Florencia | |
dc.contributor.author | Ozorio, Mónica | |
dc.contributor.author | Trinidad, Beatriz | |
dc.contributor.author | Martínez, Nidia | |
dc.contributor.author | Torales, Martha Rossana | |
dc.contributor.author | Franco, Luciano | |
dc.contributor.author | Ferreira, Luis | |
dc.contributor.author | Vera, Ninfa Isabel | |
dc.contributor.other | Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud | es |
dc.date.accessioned | 2022-04-21T01:59:38Z | |
dc.date.available | 2022-04-21T01:59:38Z | |
dc.date.issued | 2020-08-15 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14066/2979 | |
dc.description | Corresponding author at: Department of Tropical Medicine, Research Institute in Health Science (IICS-UNA), National University of Asunción, Campus UNA, San Lorenzo, Paraguay. E-mail addresses: colepuerto@hotmail.com, medicinatropical@iics.una.py. (F. del Puerto). | |
dc.description.abstract | In Paraguay, no cases of Malaria have been recorded since 2011. Microscopy and the SnM-PCR technique were implemented to detect and characterize Plasmodium spp. both in mosquitoes and in humans residing in historically endemic areas of Paraguay, to evaluate the possibility of finding asymptomatic cases and/or Plasmodium parasites circulating in anophelines. Between 2013 and 2015, 361 human blood samples were collected on filter paper, and between 2016 and 2017, 938 female Anopheles mosquitoes were captured in 15 Paraguayan localities. All the diagnostic techniques showed negative results. We observed no asymptomatic case or Plasmodium circulating in vectors. | es |
dc.description.sponsorship | Consejo Nacional de Ciencia y Tecnología | es |
dc.format.extent | 7 páginas | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.classification | 7 Salud | es |
dc.subject.other | Malaria | es |
dc.subject.other | PCR | es |
dc.subject.other | Paraguay | es |
dc.title | Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | 10.1016/j.parepi.2020.e00174 | es |
dc.description.fundingtext | Programa Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de investigación y desarrollo | es |
dc.identifier.essn | 2405-6731 | |
dc.journal.title | Parasite Epidemiology and Control | es |
dc.relation.projectCONACYT | 14-INV-463 | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.rights.copyright | © 2020 The Author(s). Published by Elsevier Ltd on behalf of World Federation of Parasitologists | |
dc.subject.ocde | 7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles | es |
dc.volume.number | 11 | es |
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