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dc.contributor.authorSchvartzman, Jorge Bernardo 
dc.contributor.authorMartínez, Víctor
dc.contributor.authorHernández, Pablo
dc.contributor.authorKrimer, Dora
dc.contributor.authorFernández de Nestosa, María José 
dc.contributor.otherUniversidad Nacional de Asunción - Facultad Politécnicaes
dc.date.accessioned2022-05-02T18:11:58Z
dc.date.available2022-05-02T18:11:58Z
dc.date.issued2021
dc.identifier.urihttp://hdl.handle.net/20.500.14066/3833
dc.description.abstractThe topology of DNA duplexes changes during replication and also after deproteinization in vitro. Here we describe these changes and then discuss for the first time how the distribution of superhelical stress affects the DNA topology of replication intermediates, taking into account the progression of replication forks. The high processivity of Topo IV to relax the left-handed (+) supercoiling that transiently accumulates ahead of the forks is not essential, since DNA gyrase and swiveling of the forks cooperate with Topo IV to accomplish this task in vivo. We conclude that despite Topo IV has a lower processivity to unlink the right-handed (+) crossings of pre-catenanes and fully replicated catenanes, this is indeed its main role in vivo. This would explain why in the absence of Topo IV replication goes-on, but fully replicated sister duplexes remain heavily catenated.es
dc.description.sponsorshipCONACYT – Consejo Nacional de Ciencia y Tecnologíaes
dc.language.isoenges
dc.subject.classification7 Saludes
dc.subject.otherCATENATIONes
dc.subject.otherDNA CHIRALITYes
dc.subject.otherDNA TOPOLOGYes
dc.subject.otherPRE-CATENATIONes
dc.subject.otherREPLICATIONes
dc.subject.otherSUPERCOILINGes
dc.titleChanges in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivoes
dc.typeresearch articlees
dc.identifier.doihttps://doi.org/10.1002/bies.202000309es
dc.description.fundingtextPROCIENCIAes
dc.journal.titleBioEssayses
dc.page.initial1es
dc.page.final7es
dc.relation.projectCONACYTPINV15-573es
dc.rights.accessRightsopen accesses
dc.rights.copyright© 2021 Wiley Periodicals LLCes


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