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Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay

dc.contributor.authorRodríguez Valinotti, María Fátima 
dc.contributor.authorNara Pereira, Eva Megumi
dc.contributor.authorMartínez Pereira, Iara Magaly 
dc.contributor.authorRodríguez Valinotti, Rosmary
dc.contributor.authorRodríguez Sánchez, Antonio
dc.contributor.otherUniversidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Saludes
dc.date.accessioned2025-10-10T12:40:44Z
dc.date.available2025-10-10T12:40:44Z
dc.date.issued2025-09-24
dc.identifier.citationRodriguez Valinotti, M. F., Nara Pereira, E. M., Martinez Pereira, M., Rodriguez Valinotti, R., & Rodriguez Sanchez, A. (2025). Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay. Viruses, 17(10), Article 1295. https://doi.org/10.3390/v17101295en
dc.identifier.otherhttps://doi.org/10.3390/v17101295es
dc.identifier.urihttp://hdl.handle.net/20.500.14066/4663
dc.descriptionCorrespondence: m.rodriguez@cedivep.com.py; Tel.: +595-984179-025 (ext. 111422).en
dc.descriptionThis article belongs to the Special Issue Bovine Viral Diarrhea Viruses and Other Pestiviruses.en
dc.description.abstractBovine viral diarrhea virus (BVDV) is the most prevalent pathogen in cattle and causes significant economic losses due to its severe clinical manifestations. It belongs to the family Flaviviridae and is distributed in species A, B, and H within the genus Pestivirus. The objective of this study was to detect and characterize BVDV using molecular techniques (RT-PCR) in semen and aborted fetuses samples that were sent to the CEDIVEP (Veterinary Diagnostic Center of Paraguay) laboratory. Seventy-three samples of semen from bulls were analyzed, and 54.7% of the samples were positive for Pestivirus A. The presence of Pestivirus A and H was detected in 2/8 spontaneously aborted fetuses. The genotypes of four individual samples of type A and four samples of type H organs were confirmed by partial sequencing of the 5-UTR region. The presence of BVDV was confirmed by molecular techniques for the first time in our country through its detection in different types of samples, as well as the presence of two genotypes. This suggests that the circulation of this virus can cause significant losses in cattle production in Paraguay.es
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnologíaes
dc.format.extent10 páginases
dc.language.isoenges
dc.publisherMultidisciplinary Digital Publishing Institutees
dc.rightsAtribución/Reconocimiento 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCría de ganadoes
dc.subjectFlaviviridaees
dc.subjectGanado bovinoes
dc.subjectPCRes
dc.subjectPestiviruses
dc.subjectPestivirus de la diarrea bovinaes
dc.subjectLivestock-raisingen
dc.subjectFlaviviridaeen
dc.subjectCattleen
dc.subjectPCRen
dc.subjectPestivirusen
dc.subjectBovine diarrhoea pestivirusen
dc.subject.otherBVDVes
dc.subject.otherParaguayes
dc.subject.otherPestivirus RT-PCRes
dc.titleMolecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguayes
dc.typeinfo:eu-repo/semantics/articlees
dc.typeinfo:eu-repo/semantics/publishedVersiones
dc.identifier.doi10.3390/v17101295es
dc.description.fundingtextPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de creación y fortalecimiento de maestrías y doctorados de excelenciaes
dc.identifier.essn1999-4915es
dc.issue.number10es
dc.journal.titleViruseses
dc.relation.projectCONACYTPOSG17-59es
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.copyright© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).es
dc.volume.number17es


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